admax system Search Results


shuttle vector admax  (Shanghai GenePharma)
90
Shanghai GenePharma shuttle vector admax
Shuttle Vector Admax, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/admax+system/shuttle+vector+admax/pmc04047337-84-20-39
Average 90 stars, based on 1 article reviews
shuttle vector admax - by Bioz Stars, 2026-10
90/100 stars
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admax-becn2  (Shanghai GenePharma)
90
Shanghai GenePharma admax-becn2
The effect of <t>BECN2</t> overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Admax Becn2, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/admax+system/admax+becn2/pmc10866406-141-5-10
Average 90 stars, based on 1 article reviews
admax-becn2 - by Bioz Stars, 2026-10
90/100 stars
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admax kit d  (Biowit Technologies)
90
Biowit Technologies admax kit d
The effect of <t>BECN2</t> overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Admax Kit D, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/admax+system/admax+kit+d/pmc04583529-35-10-13
Average 90 stars, based on 1 article reviews
admax kit d - by Bioz Stars, 2026-10
90/100 stars
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admax system  (Microbix Inc)
86
Microbix Inc admax system
The effect of <t>BECN2</t> overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Admax System, supplied by Microbix Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/admax+system/admax+system/pm41817653-169-32-34
Average 86 stars, based on 1 article reviews
admax system - by Bioz Stars, 2026-10
86/100 stars
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adenovirus admax system  (Vigene Biosciences)
86
Vigene Biosciences adenovirus admax system
The effect of <t>BECN2</t> overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.
Adenovirus Admax System, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/admax+system/adenovirus+admax+system/bio_rxiv__64898__2026__02__10__705201-19-2-5
Average 86 stars, based on 1 article reviews
adenovirus admax system - by Bioz Stars, 2026-10
86/100 stars
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Image Search Results


The effect of BECN2 overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Aging (Albany NY)

Article Title: Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

doi: 10.18632/aging.205386

Figure Lengend Snippet: The effect of BECN2 overexpression on the function of LPS-induced ATDC5 degeneration. ( A ) The mRNA level of BECN2 was detected by RT-PCR in LPS-induced ATDC5 cells. ( B ) The effect of ADmax-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( C ) The protein level of BECN2 was evaluated by western blot. ( D ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( E ) CCK-8 was used to assess cell viability. ( F , G ) Cell apoptosis was detected by Muse. ( H ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( I , J ) Autophagy-related protein expression levels were determined by immunoblotting. ( K , L ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: For adenovirus transfection, ADMax-NC and ADMax-BECN2 were constructed by the Shanghai Genepharma Company (Genepharma, China).

Techniques: Over Expression, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Software, CCK-8 Assay, Immunofluorescence, Staining, In Vitro

Increased BECN2 attenuated the autophagy and inflammation of LPS-induced ATDC5 degeneration. ( A ) Western blot was employed to detect the expression of ATG14, VPS34 and GASP1. ( B ) The protein expression of ATG14, VPS34 and GASP1 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( C ) ATG14, VPS34 and GASP1 expression were determined by immunofluorescence staining (scale bar: 100μm). ( D – F ) Average optical density was calculated by ImageJ software. ( G ) Western blot was employed to detect the expression of NLRP3, NLRC4, NLRP1 and AIM2. ( H ) The protein expression of NLRP3, NLRC4, NLRP1 and AIM2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( I ) NLRP3, NLRC4, NLRP1 and AIM2 expression were determined by immunofluorescence staining (scale bar: 100μm). ( J – M ) Average optical density was calculated by ImageJ software. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Aging (Albany NY)

Article Title: Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

doi: 10.18632/aging.205386

Figure Lengend Snippet: Increased BECN2 attenuated the autophagy and inflammation of LPS-induced ATDC5 degeneration. ( A ) Western blot was employed to detect the expression of ATG14, VPS34 and GASP1. ( B ) The protein expression of ATG14, VPS34 and GASP1 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( C ) ATG14, VPS34 and GASP1 expression were determined by immunofluorescence staining (scale bar: 100μm). ( D – F ) Average optical density was calculated by ImageJ software. ( G ) Western blot was employed to detect the expression of NLRP3, NLRC4, NLRP1 and AIM2. ( H ) The protein expression of NLRP3, NLRC4, NLRP1 and AIM2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( I ) NLRP3, NLRC4, NLRP1 and AIM2 expression were determined by immunofluorescence staining (scale bar: 100μm). ( J – M ) Average optical density was calculated by ImageJ software. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: For adenovirus transfection, ADMax-NC and ADMax-BECN2 were constructed by the Shanghai Genepharma Company (Genepharma, China).

Techniques: Western Blot, Expressing, Software, Immunofluorescence, Staining, In Vitro

The effect of BECN2 knockdown on the function of ATDC5. ( A ) The effect of siRNA-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( B ) The protein level of BECN2 was evaluated by western blot. ( C ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( D ) CCK-8 was used to assess cell viability. ( E , F ) Cell apoptosis was detected by Muse. ( G ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( H , I ) Autophagy-related protein expression levels were determined by immunoblotting. ( J , K ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Aging (Albany NY)

Article Title: Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

doi: 10.18632/aging.205386

Figure Lengend Snippet: The effect of BECN2 knockdown on the function of ATDC5. ( A ) The effect of siRNA-BECN2 on the mRNA expression of BECN2 was detected by RT-PCR. ( B ) The protein level of BECN2 was evaluated by western blot. ( C ) The protein expression of BECN2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( D ) CCK-8 was used to assess cell viability. ( E , F ) Cell apoptosis was detected by Muse. ( G ) The immunofluorescence staining of LC3 was used to evaluate the autophagy level (green signals represent LC3, blue signals represent DAPI, scale bar: 10 μm). ( H , I ) Autophagy-related protein expression levels were determined by immunoblotting. ( J , K ) The expression of IL-1β, Caspase1 and IL-18 were assessed by western blot. Relative protein expression was qualified by ImageJ software, GAPDH was used as the internal control, respectively. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: For adenovirus transfection, ADMax-NC and ADMax-BECN2 were constructed by the Shanghai Genepharma Company (Genepharma, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software, CCK-8 Assay, Immunofluorescence, Staining, In Vitro

Decreased BECN2 activated the autophagy and inflammation of ATDC5. ( A ) Western blot was employed to detect the expression of ATG14, VPS34 and GASP1. ( B ) The protein expression of ATG14, VPS34 and GASP1 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( C ) ATG14, VPS34 and GASP1 expression were determined by immunofluorescence staining (scale bar: 100μm). ( D – F ) Average optical density was calculated by ImageJ software. ( G ) Western blot was employed to detect the expression of NLRP3, NLRC4, NLRP1 and AIM2. ( H ) The protein expression of NLRP3, NLRC4, NLRP1 and AIM2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( I ) NLRP3, NLRC4, NLRP1 and AIM2 expression were determined by immunofluorescence staining (scale bar: 100μm). ( J – M ) Average optical density was calculated by ImageJ software. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Aging (Albany NY)

Article Title: Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation

doi: 10.18632/aging.205386

Figure Lengend Snippet: Decreased BECN2 activated the autophagy and inflammation of ATDC5. ( A ) Western blot was employed to detect the expression of ATG14, VPS34 and GASP1. ( B ) The protein expression of ATG14, VPS34 and GASP1 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( C ) ATG14, VPS34 and GASP1 expression were determined by immunofluorescence staining (scale bar: 100μm). ( D – F ) Average optical density was calculated by ImageJ software. ( G ) Western blot was employed to detect the expression of NLRP3, NLRC4, NLRP1 and AIM2. ( H ) The protein expression of NLRP3, NLRC4, NLRP1 and AIM2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). ( I ) NLRP3, NLRC4, NLRP1 and AIM2 expression were determined by immunofluorescence staining (scale bar: 100μm). ( J – M ) Average optical density was calculated by ImageJ software. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: For adenovirus transfection, ADMax-NC and ADMax-BECN2 were constructed by the Shanghai Genepharma Company (Genepharma, China).

Techniques: Western Blot, Expressing, Software, Immunofluorescence, Staining, In Vitro